P22 phage capsids under pressure
نویسندگان
چکیده
منابع مشابه
Implementation of p22 viral capsids as nanoplatforms.
Viral capsids are dynamic macromolecular machines which self-assemble and undergo concerted conformational changes during their life cycle. We have taken advantage of the inherent structural flexibility of viral capsids and generated two morphologically different types of viral nanoplatforms from the bacteriophage P22 capsids. Their interior surfaces were genetically manipulated for site-specif...
متن کاملMutations of phage P22 affecting phage DNA synthesis and lysogenization.
Two temperature-sensitive mutants of phage P22, ts18.1 and ts12.1, are described which have recessive DNA-negative phenotypes. At high temperature they fail to synthesize phage DNA. In mixed infection with the two mutants or either mutant and wild-type phage, DNA synthesis and phage production are normal. In addition, under non-permissive conditions, these mutants fail to lysogenize and show do...
متن کاملP22 viral capsids as nanocomposite high-relaxivity MRI contrast agents.
Attachment of multiple chelated Gd(3+) ions to the interior of bacteriophage P22 viral capsids affords nanoscale MRI contrast agents with extremely high relaxivity values. Highly fenestrated "wiffleball" morphology is unique to P22 and assures water exchange between the environment and interior cavity of the capsid. The cavity of P22 "wiffleball" was functionalized with a branched oligomer comp...
متن کاملRecombinational circularization of Salmonella phage P22 DNA.
The development of phage P22 following infection involves a mandatory recombination step, the timing of which is the same in both the lytic and lysogenic pathways. Covalently circular molecules of phage DNA were identified by alkaline sucrose sedimentation of infected cell lysates. The time of appearance of these structures corresponds to the time of the essential recombination step. On infecti...
متن کاملSpecialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. I. Transduction of R factor 222 by phage P22.
Transduction of R factor 222, which carries four drug resistance markers (~0, strR, camR, totR), by phage P22 in Salmonella typhimurium results in the segregation of the drug resistance markers into tetR and the other three. The tetR marker was found to be integrated at proA near the P22 prophage attachment site of the recipient chromosome, confirming the previous result of Dubnau and Stocker (...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1994
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(94)80919-1